Carlier C, Courvalin P
J Antibiot (Tokyo). 1982 May;35(5):629-34. doi: 10.7164/antibiotics.35.629.
Bacillus circulans NRRL B-3312 produces the aminoglycoside antibiotic butirosin and encodes an aminoglycoside 3'-phosphotransferase. We detected a 48 kilobase plasmid, pIP850, in this strain; this was analyzed by agarose gel electrophoresis following digestion with EcoRI restriction endonuclease and by nucleic acid hybridization. The results obtained indicate that plasmid pIP850 does not carry the structural gene for the aminoglycoside modifying enzyme.
环状芽孢杆菌NRRL B-3312可产生氨基糖苷类抗生素布替罗星,并编码一种氨基糖苷3'-磷酸转移酶。我们在该菌株中检测到一个48千碱基的质粒pIP850;用EcoRI限制性内切酶消化后通过琼脂糖凝胶电泳以及核酸杂交对其进行了分析。所得结果表明质粒pIP850不携带氨基糖苷修饰酶的结构基因。