一种研究肾单位和集合小管显微解剖学的全新且简便的方法。

A new and simple method for studying the microanatomy of nephrons and collecting tubules.

作者信息

Ojeda J L, Garcia-Porrero J A, Ros M A

出版信息

Stain Technol. 1984 Jan;59(1):37-42. doi: 10.3109/10520298409113826.

DOI:10.3109/10520298409113826

PMID:6206621

Abstract

A simple procedure for studying the microanatomy of the kidney by light microscopy is described. Specimens are dried by the critical-point method and later impregnated with a mounting medium. The retention of air within the lumen of nephrons and collecting tubules permits their visualization. The method allows the observation of renal microanatomy without the distortions produced by microdissection or the loss of structural relationships among different kidney components. It also permits observation of the same specimen by both light and scanning electron microscopy.

摘要

本文描述了一种通过光学显微镜研究肾脏微观解剖结构的简单方法。标本采用临界点干燥法干燥，随后用封固介质进行浸渍。肾单位和集合小管腔内空气的保留使得它们能够被观察到。该方法能够观察肾脏微观解剖结构，而不会出现显微解剖所产生的变形或不同肾脏成分之间结构关系的丧失。它还允许通过光学显微镜和扫描电子显微镜对同一标本进行观察。