非人类α-巨球蛋白的圆二色光谱

Circular dichroic spectroscopy of non-human alpha-macroglobulins.

作者信息

Feldman S R, Pizzo S V

出版信息

Biochem Biophys Res Commun. 1984 Sep 17;123(2):771-7. doi: 10.1016/0006-291x(84)90296-1.

PMID:6207818

Abstract

Bovine, chicken and frog alpha-macroglobulins and ovomacroglobulin were studied by circular dichroic spectroscopy over the region 205-250 nm. All four spectra exhibited negative ellipticity with minima at about 215 nm similar to that reported for human alpha 2-macroglobulin. On reaction of the alpha-macroglobulins with trypsin, the spectrum of each of the four changed similarly. However, these proteins exhibited different conformational changes when treated with methylamine. These differences were exploited to determine which characteristics of alpha-macroglobulins correlate with changes in circular dichroic spectroscopy.

摘要

利用圆二色光谱法对牛、鸡和青蛙的α-巨球蛋白以及卵巨球蛋白在205 - 250纳米区域进行了研究。所有这四种蛋白质的光谱在约215纳米处均呈现负椭圆率最小值，这与报道的人α2-巨球蛋白相似。α-巨球蛋白与胰蛋白酶反应时，这四种蛋白质各自的光谱变化相似。然而，用甲胺处理时，这些蛋白质呈现出不同的构象变化。利用这些差异来确定α-巨球蛋白的哪些特性与圆二色光谱的变化相关。