Bielefeldt Ohmann H, Filion L G, Babiuk L A
Vet Immunol Immunopathol. 1984 Jul;6(3-4):307-20. doi: 10.1016/0165-2427(84)90056-4.
Previously we have shown that macrophages acted as accessory cells for the induction of bovine suppressor cells with concanavalin A (Con A) and in mitogen induced lymphocyte proliferation. In exploration of possible mechanisms for the lymphocyte-macrophage interaction, it was found that the requirement for macrophages could not be substituted for with 2-mercaptoethanol. Furthermore, direct contact between lymphocytes and macrophages was necessary for optimal generation of suppressor cells. However, de novo protein synthesis by macrophages or lymphocytes apparently was not essential for the induction of Con A suppressor cells. For generation of suppressor cells, Con A had to be present in a soluble form during the entire induction period, as mitogen "pulsing" of either lymphocytes or macrophages prior to cocultivation did not lead to generation of suppressor activity.
先前我们已经表明，巨噬细胞在伴刀豆球蛋白A（Con A）诱导牛抑制细胞以及在有丝分裂原诱导的淋巴细胞增殖过程中作为辅助细胞发挥作用。在探索淋巴细胞 - 巨噬细胞相互作用的可能机制时，发现巨噬细胞的需求不能被2 - 巯基乙醇替代。此外，淋巴细胞与巨噬细胞之间的直接接触对于抑制细胞的最佳产生是必要的。然而，巨噬细胞或淋巴细胞从头合成蛋白质显然对于Con A抑制细胞的诱导不是必需的。为了产生抑制细胞，在整个诱导期Con A必须以可溶形式存在，因为在共培养之前对淋巴细胞或巨噬细胞进行有丝分裂原“脉冲处理”不会导致抑制活性的产生。
