烟草叶绿体中4.5S和5S RNA基因的克隆与特性分析

Cloning and characterization of 4.5S and 5S RNA genes in tobacco chloroplasts.

作者信息

Takaiwa F, Sugiura M

出版信息

Gene. 1980 Jul;10(2):95-103. doi: 10.1016/0378-1119(80)90127-4.

Abstract

Tobacco chloroplast 4.5S and 5S RNAs were shown to hybridize with a 0.9 . 10(6) dalton EcoRI fragment of tobacco chloroplast DNA. Recombinant plasmids were constructed from fragments produced by partial digestion of the chloroplast DNA with EcoRI and the pMB9 plasmid as a vector. Five recombinants containing the 4.5S and 5S genes were selected by the colony hybridization technique. One of these plasmids contained also the 16S and 23S RNA genes and was mapped using several restriction endonucleases as well as DNA-RNA hybridization. The order of rRNA genes is 16S-23S-4.5S-5S and the four rRNA genes are coded for by the same DNA strand.

摘要

烟草叶绿体4.5S和5S RNA已被证明能与烟草叶绿体DNA的一个0.9×10⁶道尔顿的EcoRI片段杂交。用EcoRI对叶绿体DNA进行部分酶切产生片段，并以pMB9质粒为载体构建重组质粒。通过菌落杂交技术筛选出五个含有4.5S和5S基因的重组体。其中一个质粒还含有16S和23S RNA基因，并使用几种限制性内切酶以及DNA-RNA杂交技术进行了图谱绘制。rRNA基因的顺序是16S-23S-4.5S-5S，这四个rRNA基因由同一条DNA链编码。