Grisvard J, Tuffet-Anghileri A
Nucleic Acids Res. 1980 Jun 25;8(12):2843-58. doi: 10.1093/nar/8.12.2843.
Total DNA from Cucumis melo contains a 1.706 satellite DNA which can be resolved into two components; one of these components has a higher temperature of melting (component I) then the other component II). In this study, we have further investigated these components by thermal denaturation and by Eco R1 digestion. Component I reveals a homogeneous melting profile and is only partially cleaved by Eco R1, whereas component II reveals a heterogeneous melting profile and is entirely digested by Eco R1. A possible mitochondrial origin for component II is discussed. When an in vitro culture of root tissues from Cucumis melo was initiated or when the phytohormone (NAA) concentration of established cultures was modified, a new satellite DNA (1.719) appears transitorily and the satellite DNAs already present in seedlings undergo quantitative and possibly qualitative variations. Satellite DNAs therefore seem to be involved in the response to some physiological variations.
甜瓜的总DNA包含一种1.706卫星DNA,它可分解为两个组分;其中一个组分(组分I)的解链温度高于另一个组分(组分II)。在本研究中,我们通过热变性和Eco R1酶切进一步研究了这些组分。组分I呈现均匀的解链图谱,仅被Eco R1部分切割,而组分II呈现异质的解链图谱,并被Eco R1完全消化。讨论了组分II可能的线粒体起源。当启动甜瓜根组织的体外培养或改变已建立培养物的植物激素(NAA)浓度时,一种新的卫星DNA(1.719)会短暂出现,并且幼苗中已存在的卫星DNA会发生数量变化,也可能发生质量变化。因此,卫星DNA似乎参与了对某些生理变化的响应。