Glaser G, Kobi S, Oppenheim A B
Nucleic Acids Res. 1980 Oct 10;8(19):4327-35. doi: 10.1093/nar/8.19.4327.
A Lambda phage was constructed in which the structural gene for beta galactosidase is fused to a DNA segment carrying the ribosomal promoter rrnB of E. coli. In this hybrid operon beta galactosidase synthesis in vitro is repressed by ppGpp. Repression of beta galactosidase synthesis by cAMP is reported.
构建了一种λ噬菌体,其中β-半乳糖苷酶的结构基因与携带大肠杆菌核糖体启动子rrnB的DNA片段融合。在这个杂交操纵子中,体外β-半乳糖苷酶的合成受到ppGpp的抑制。有报道称cAMP可抑制β-半乳糖苷酶的合成。