通过限制酶分析测定灵长类核糖体RNA基因的差异。

Divergence of primate ribosomal RNA genes as assayed by restriction enzyme analysis.

作者信息

Nelkin B, Strayer D, Vogelstein B

出版信息

Gene. 1980 Oct;11(1-2):89-96. doi: 10.1016/0378-1119(80)90089-x.

Abstract

Primate ribosomal RNA (rRNA) genes have been compared by restriction endonuclease mapping. In all species examined, the restriction map of the reiterated ribosomal DNA is simple (within the limits of detection by hybridization with rRNA) and is consistent with a high degree of homogeneity among the repeats. Within a species, all members have similar rDNA restriction patterns. However, different species of primates have distinctly different rDNA restriction maps; even chimpanzee and man can be discerned by their rDNA restriction patterns. Possible mechanisms for maintenance of homogeneity of the rDNA repeats within a species, while allowing divergence among closely related species, are discussed.

摘要

已通过限制性内切酶图谱分析对灵长类动物的核糖体RNA（rRNA）基因进行了比较。在所有检测的物种中，重复核糖体DNA的限制性图谱很简单（在与rRNA杂交检测的限度内），并且与各重复序列之间的高度同质性相符。在一个物种内，所有成员都有相似的rDNA限制性图谱。然而，不同的灵长类物种有明显不同的rDNA限制性图谱；甚至黑猩猩和人类也可以通过它们的rDNA限制性图谱区分开来。本文讨论了在一个物种内维持rDNA重复序列同质性，同时允许密切相关物种之间存在差异的可能机制。