溶组织内阿米巴质膜中的血清蛋白。

Serum proteins in the plasma membrane of Entamoeba histolytica.

作者信息

Noya González O, Warren L G, Gohd R

出版信息

Arch Invest Med (Mex). 1980;11(1 Suppl):109-14.

Abstract

The ability of axenic Entamoeba histolytica, strain 200:NIH, to adsorb serum proteins was studied. Amebae were grown in Diamond's TPS-1 medium. Serological reactivity was observed between the plasma membrane of E. histolytica and rabbit anti-bovine or anti-human serum when the amebae were grown, respectively, with bovine or human serum. Antisera were obtained from rabbits immunized with culture medium, amebal homogenates, and various amebal subcellular fractions: soluble phase, microsomal fraction, and a large vesicle fraction. The presence of serum proteins was demonstrated in the large vesicle fraction by the double diffusion and indirect hemagglutination tests. Immunofluorescence (IF) studies on amebae grown in bovine or human serum showed the surface amebae exhibited IF with specific antibodies against the serum in which they were grown. Rabbit anti-human serum blocked IF on the surface of amebae grown with human serum. From these data we conclude that the plasma membrane of in vitro grown E. histolytica can adsorb human and bovine serum proteins.

摘要

对无菌溶组织内阿米巴200:NIH株吸附血清蛋白的能力进行了研究。阿米巴在戴蒙德TPS - 1培养基中培养。当溶组织内阿米巴分别与牛血清或人血清一起培养时，观察到其质膜与兔抗牛血清或抗人血清之间的血清学反应性。抗血清取自用培养基、阿米巴匀浆以及各种阿米巴亚细胞组分（可溶性相、微粒体组分和大囊泡组分）免疫的兔子。通过双向扩散和间接血凝试验在大囊泡组分中证实了血清蛋白的存在。对在牛血清或人血清中培养的阿米巴进行的免疫荧光（IF）研究表明，表面阿米巴与针对其生长所用血清的特异性抗体呈现免疫荧光。兔抗人血清阻断了用人血清培养的阿米巴表面的免疫荧光。从这些数据我们得出结论，体外培养的溶组织内阿米巴的质膜能够吸附人血清和牛血清蛋白。