猴空泡病毒 40（SV40）转化的猿猴细胞支持早期 SV40 突变体的复制。

SV40-transformed simian cells support the replication of early SV40 mutants.

作者信息

Gluzman Y

出版信息

Cell. 1981 Jan;23(1):175-82. doi: 10.1016/0092-8674(81)90282-8.

Abstract

CV-1, an established line of simian cells permissive for lytic growth of SV40, were transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen. Three transformed lines (COS-1, -3, -7) were established and found to contain T antigen; retain complete permissiveness for lytic growth of SV40; support the replication of tsA209 virus at 40 degrees C; and support the replication of pure populations of SV40 mutants with deletions in the early region. One of the lines (COS-1) contains a single integrated copy of the complete early region of SV40 DNA. These cells are possible hosts for the propagation of pure populations of recombinant SV40 viruses.

摘要

CV-1是一种已建立的猿猴细胞系，对SV40的裂解生长具有允许性，它被编码野生型T抗原的SV40起源缺陷型突变体转化。建立了三个转化细胞系（COS-1、-3、-7），发现它们含有T抗原；对SV40的裂解生长保持完全允许性；支持tsA209病毒在40℃下复制；并支持早期区域有缺失的SV40突变体纯群体的复制。其中一个细胞系（COS-1）含有SV40 DNA完整早期区域的单个整合拷贝。这些细胞可能是重组SV40病毒纯群体繁殖的宿主。