Abdukarimov A, Guliamova T G, Aripdzhanov A A, Khan M Z
Probl Endokrinol (Mosk). 1981 Mar-Apr;27(2):66-70.
The work concerns a new approach to evaluating the receptor and hormone-receptor complex specific interaction with DNA and revealing the cause of the absence of the hormone-receptor complex acceptation with HeLa cell chromosomes. It has been shown that hormone-receptor complexing should precede specific interaction with DNA. The purified receptor-thyroxin complex is saturatable and specifically competes with Hae III restrictase, protecting specific sites of DNA recognition from this enzyme. Specificity of the hormone-receptor complex interaction with DNA depends on the sequences surrounding Hae III restriction site. The HeLa cell hormone-receptor complex does not interact with the recognition site for the normal hormone-receptor complex.