氯霉素简易放射酶测定法
Simplified radioenzymatic assay for chloramphenicol.
作者信息
Robison L R, Seligsohn R, Lerner S A
出版信息
Antimicrob Agents Chemother. 1978 Jan;13(1):25-9. doi: 10.1128/AAC.13.1.25.
Abstract
A simplified radioenzymatic assay for chloramphenicol was developed by eliminating the need for cumbersome extraction procedures. After the acetylation of chloramphenicol with [(14)C]acetyl coenzyme A in the presence of chloramphenicol acetyltransferase, the reaction mixture was added to a toluene-based scintillation fluid. Since (14)C-acetylated chloramphenicol is more soluble than [(14)C]acetyl coenzyme A in toluene, the radioactive product could be counted directly. The rapidity of this assay, as well as its accuracy, precision, and specificity, makes it particularly suitable for clinical use. In contrast to previous reports of enzymatic assays for chloramphenicol, we have found that results of the assay of standards prepared in serum were up to 25% higher than those of standards prepared in saline, cerebrospinal fluid, or urine.
摘要
通过省去繁琐的提取步骤，开发了一种用于氯霉素的简化放射酶测定法。在氯霉素乙酰转移酶存在的情况下，用[¹⁴C]乙酰辅酶A对氯霉素进行乙酰化后，将反应混合物加入基于甲苯的闪烁液中。由于¹⁴C-乙酰化氯霉素在甲苯中的溶解度比[¹⁴C]乙酰辅酶A高，放射性产物可以直接计数。该测定法的快速性及其准确性、精密度和特异性使其特别适用于临床应用。与之前关于氯霉素酶法测定的报道相反，我们发现血清中制备的标准品的测定结果比盐溶液、脑脊液或尿液中制备的标准品的测定结果高出25%。
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本文引用的文献
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