Thomson J A, Hendson M, Magnes R M
J Bacteriol. 1981 Oct;148(1):374-8. doi: 10.1128/jb.148.1.374-378.1981.
A halotolerant, collagenolytic strain of Vibrio sp. was conjugated with an Escherichia coli strain carrying plasmid RP4. The plasmid was transferred to and maintained in the Vibrio and could be subsequently transferred in matings to suitably marked stains of the same species. After conjugation with an E. coli carrying the cointegrate plasmid RP4::Mu cts61::Tn7, Vibrio transconjugants were selected that carried Tn7 inserted into the bacterial chromosome. A large proportion of these transconjugants were auxotrophic, showing that plasmid suicide by Mu can be used to isolate Tn7-derived mutants in Vibrio. Approximately half of the auxotrophs isolate Tn7-derived mutants in Vibrio. Approximately half of the auxotrophs isolated were ilv mutants, all of which exhibited the same phenotype. Thus, although Tn7 insertion can induce auxotrophy, including trp, thy, his and ura, in Vibrio, there does appear to be a hot spot for integration in the ilv operon.
一株耐盐、能产生胶原酶的弧菌属菌株与携带质粒RP4的大肠杆菌菌株进行了接合。该质粒被转移至弧菌中并能在其中维持，随后还能在交配过程中转移至同一物种的合适标记菌株中。与携带共整合质粒RP4::Mu cts61::Tn7的大肠杆菌接合后，筛选出了携带插入到细菌染色体中的Tn7的弧菌转接合子。这些转接合子中很大一部分是营养缺陷型的，这表明Mu介导的质粒自杀可用于在弧菌中分离Tn7衍生的突变体。分离出的营养缺陷型中约一半是ilv突变体，它们都表现出相同的表型。因此，虽然Tn7插入可在弧菌中诱导营养缺陷型，包括色氨酸、胸腺嘧啶、组氨酸和尿嘧啶营养缺陷型，但ilv操纵子中似乎存在一个整合热点。
