枯草芽孢杆菌噬菌体SPβ c1是SPβ的缺失突变体。

Bacillus subtilis bacteriophages SP beta c1 is a deletion mutant of SP beta.

作者信息

Fink P S, Korman R Z, Odebralski J M, Zahler S A

出版信息

Mol Gen Genet. 1981;182(3):514-5. doi: 10.1007/BF00293946.

PMID:6272067

Abstract

The restriction fragment patterns of two mutants forms of the temperate Bacillus subtilis bacteriophage SP beta have been examined. The DNA of a heat-inducible mutant, SP beta c2, which has a molecular size of 128 kilobases (kb), yields the same restriction pattern as the wild type SP beta c+ DNA. The DNA of a clear-plaque mutant, SP beta c1, has a molecular size of 117 kb, and is deleted for an 11 kb region of phage DNA. Neither SP beta c1 nor SP beta c2 DNA is cleaved by the endonuclease HaeIII.

摘要

已对温和型枯草芽孢杆菌噬菌体SPβ的两种突变体形式的限制性片段模式进行了检测。热诱导突变体SPβ c2的DNA分子大小为128千碱基（kb），其限制性模式与野生型SPβ c+ DNA相同。清晰噬菌斑突变体SPβ c1的DNA分子大小为117 kb，噬菌体DNA的一个11 kb区域缺失。SPβ c1和SPβ c2的DNA均不能被核酸内切酶HaeIII切割。