Fuhrman S A, Deininger P L, LaPorte P, Friedmann T, Geiduschek E P
Nucleic Acids Res. 1981 Dec 11;9(23):6439-56. doi: 10.1093/nar/9.23.6439.
A series of clones that contain human Alu family elements are actively transcribed in soluble in vitro RNA polymerase III systems. The 5' ends of the in vitro transcripts are located about 170 nucleotides upstream of the eponymous Alu I site of the repeat, while a region associated with specifying of the initiation site for in vitro transcription lies in the region between 79 and 106 nucleotides upstream of the central Alu site. Thus, the RNA polymerase III transcription unit defined by the human Alu family is similar to other RNA polymerase III transcription units in possessing an internal region that is required for active transcription in vitro.
一系列含有人类Alu家族元件的克隆在可溶性体外RNA聚合酶III系统中被积极转录。体外转录物的5′端位于重复序列中同名Alu I位点上游约170个核苷酸处，而与体外转录起始位点特异性相关的区域位于中央Alu位点上游79至106个核苷酸之间的区域。因此，由人类Alu家族定义的RNA聚合酶III转录单位与其他RNA聚合酶III转录单位相似，都拥有一个体外活性转录所需的内部区域。
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