Crance J M, Deloince R, Corbet G, Fontanges R
C R Seances Acad Sci III. 1981 Dec 7;293(13):693-6.
Hepatitis A virus was purified from on early acute phase human stool by a procedure using 10% polyethylene glycol precipitation, sepharose 2B gel filtration, isopycnic banding in cesium chloride, rate zonal separation in sucrose - 99,5% of the 280 nm absorbing fecal impurities were removed.
采用10%聚乙二醇沉淀、琼脂糖2B凝胶过滤、氯化铯等密度梯度离心以及蔗糖速率区带分离,去除了99.5%在280nm处有吸收的粪便杂质。