用于质粒载体pBR322中DNA测序的一组合成寡脱氧核糖核苷酸引物。

A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322.

作者信息

Wallace R B, Johnson M J, Suggs S V, Miyoshi K, Bhatt R, Itakura K

出版信息

Gene. 1981 Dec;16(1-3):21-6. doi: 10.1016/0378-1119(81)90057-3.

Abstract

Seven oligonucleotide primers complementary to the plasmid vector pBR322 at positions adjacent to five of the unique restriction endonuclease cleavage sites (EcoRI, HindIII, BamHI, SalI and PstI) have been chemically synthesized. The polarity of the primers is such that any DNA inserted at one or a combination of two of the above restriction sites may be sequenced by the chain termination method using one of the synthetic DNA primers. One of the primers for sequencing inserts at the PstI site of pBR322 is also complementary to the M13 phage vector designated bla6. This set of universal primers is useful for rapid sequence determination of DNA cloned into pBR322 or M13bla6.

摘要

已化学合成了七条寡核苷酸引物，它们与质粒载体pBR322上五个独特的限制性内切酶切割位点（EcoRI、HindIII、BamHI、SalI和PstI）中五个位点相邻位置互补。引物的极性使得插入上述一个或两个限制性位点组合处的任何DNA都可以使用其中一种合成DNA引物通过链终止法进行测序。用于对插入pBR322的PstI位点进行测序的引物之一也与命名为bla6的M13噬菌体载体互补。这组通用引物可用于快速测定克隆到pBR322或M13bla6中的DNA序列。