兔和山羊对山羊关节炎-脑炎病毒的中和抗体反应。
Neutralizing antibody response of rabbits and goats to caprine arthritis-encephalitis virus.
作者信息
Klevjer-Anderson P, McGuire T C
出版信息
Infect Immun. 1982 Nov;38(2):455-61. doi: 10.1128/iai.38.2.455-461.1982.
Rabbits were immunized with purified caprine arthritis-encephalitis virus and examined for neutralizing activity. Analysis of virus-antiserum interaction at 37 degrees C demonstrated little loss of viral infectivity after incubation with heat-inactivated rabbit antiserum for 60 min. However, sensitization of virus (as assessed by the addition of complement) occurred almost immediately and was 95% complete after 10 min. The complement-dependent neutralizing activity was associated with the immunoglobulin G fraction of rabbit antiserum. Addition of goat anti-rabbit immunoglobulin G to the immune rabbit serum-caprine arthritis-encephalitis virus mixture also resulted in neutralization of infectivity when unbound antibody was removed before addition of the anti-immunoglobulin. Serum from most caprine arthritis-encephalitis virus-infected goats contains antibody activity to the core protein p28, as demonstrated by immunodiffusion and enzyme-linked immunosorbent assay. However, attempts to demonstrate neutralizing activity in the serum of goats up to 1.5 years post-inoculation or in serum of hyperimmunized goats were unsuccessful when the sera were examined alone or in combination with complement or rabbit anti-goat immunoglobulin or both.
用纯化的山羊关节炎-脑炎病毒免疫兔子，并检测其中和活性。在37℃分析病毒-抗血清相互作用，结果显示热灭活的兔抗血清与病毒孵育60分钟后，病毒感染力几乎没有损失。然而，病毒致敏（通过添加补体评估）几乎立即发生，10分钟后95%完成。补体依赖性中和活性与兔抗血清的免疫球蛋白G组分相关。在添加抗免疫球蛋白之前去除未结合的抗体，向免疫兔血清-山羊关节炎-脑炎病毒混合物中添加山羊抗兔免疫球蛋白G也会导致感染性中和。免疫扩散和酶联免疫吸附测定表明，大多数感染山羊关节炎-脑炎病毒的山羊血清中含有针对核心蛋白p28的抗体活性。然而，单独检测接种后长达1.5年的山羊血清或超免疫山羊血清中的中和活性，或与补体或兔抗山羊免疫球蛋白或两者结合检测时，均未成功。
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