Yasuda S, Takagi T
J Bacteriol. 1983 Jun;154(3):1153-61. doi: 10.1128/jb.154.3.1153-1161.1983.
A derivative of the runaway-replication plasmid was constructed. This plasmid, pSY343, has the gene for kanamycin resistance and single sites for EcoRI, BamHI, HindIII, KpnI, and XhoI that can be used as cloning sites without inactivating the kanamycin resistance gene or the replication genes. Three replication genes of Escherichia coli were cloned on the plasmid. The activity of dnaA, dnaZ, and ssb gene products were 200-, 90-, and 60-fold greater, respectively, in the cells containing these plasmids than in normal cells.
构建了一种失控复制质粒的衍生物。该质粒pSY343具有卡那霉素抗性基因以及EcoRI、BamHI、HindIII、KpnI和XhoI的单一位点,这些位点可用作克隆位点,而不会使卡那霉素抗性基因或复制基因失活。将大肠杆菌的三个复制基因克隆到了该质粒上。与正常细胞相比,含有这些质粒的细胞中dnaA、dnaZ和ssb基因产物的活性分别高出200倍、90倍和60倍。