Dyall-Smith M L, Elleman T C, Hoyne P A, Holmes I H, Azad A A
Nucleic Acids Res. 1983 May 25;11(10):3351-62. doi: 10.1093/nar/11.10.3351.
The genome of the UK bovine rotavirus, which consists of eleven segments of dsRNA was polyadenylated and reverse-transcribed into cDNA. Complementary cDNA strands were annealed and the termini of the duplexes completed using DNA polymerase I. Full-length DNA copies of RNA segments 7, 8 and 9 were cloned into the Pst I site of pBR322 and a clone containing the entire gene 7 was identified and sequenced. Gene 7 is 1059 nucleotides in length and contains a single long open reading frame capable of coding for a protein of 317 amino-acids. The known gene product of segment 7 is a protein with an estimated molecular weight of 33,000 daltons. When the UK bovine rotavirus gene 7 sequence was compared with the published data for the homologous gene (segment 8) of the simian rotavirus SA11, it was found to be identical to it in size and the arrangement of the proposed coding and non-coding regions, and very similar in nucleotide sequence (88% homology). Most of the base changes are silent and the predicted amino-acid sequences are almost identical (96% homology).
英国牛轮状病毒的基因组由11条双链RNA片段组成,这些片段经过聚腺苷酸化处理后被逆转录成cDNA。互补的cDNA链进行退火处理,并使用DNA聚合酶I完成双链的末端处理。RNA片段7、8和9的全长DNA拷贝被克隆到pBR322的Pst I位点,鉴定并测序了一个包含完整基因7的克隆。基因7长度为1059个核苷酸,包含一个单一的长开放阅读框,能够编码一个由317个氨基酸组成的蛋白质。片段7的已知基因产物是一种估计分子量为33000道尔顿的蛋白质。当将英国牛轮状病毒基因7的序列与猿猴轮状病毒SA11同源基因(片段8)的已发表数据进行比较时,发现其在大小以及提议的编码区和非编码区的排列上与之一致,并且在核苷酸序列上非常相似(88%同源性)。大多数碱基变化是沉默的,预测的氨基酸序列几乎相同(96%同源性)。