Boswell R E, Jahn C L, Greslin A F, Prescott D M
Nucleic Acids Res. 1983 Jun 11;11(11):3651-63. doi: 10.1093/nar/11.11.3651.
In order to study the derivation of the macronuclear genome from the micronuclear genome in Oxytricha nova micronuclear DNA was partially digested with EcoRI, size fractionated, and then cloned in the lambda phage Charon 8. Clones were selected a) at random b) by hybridization with macronuclear DNA or c) by hybridization with clones of macronuclear DNA. One group of these clones contains only unique sequence DNA, and all of these had sequences that were homologous to macronuclear sequences. The number of macronuclear genes with sequences homologous to these micronuclear clones indicates that macronuclear sequences are clustered in the micronuclear genome. Many micronuclear clones contain repetitive DNA sequences and hybridize to numerous EcoRI fragments of total micronuclear DNA, yielding similar but non-identical patterns. Some micronuclear clones containing these repetitive sequences also contained unique sequence DNA that hybridized to a macronuclear sequence. These clones define a major interspersed repetitive sequence family in the micronuclear genome that is eliminated during formation of the macronuclear genome.
为了研究新大核草履虫大核基因组源自小核基因组的情况，用EcoRI对新大核草履虫的小核DNA进行部分酶切，按大小分级分离，然后克隆到λ噬菌体Charon 8中。通过以下方式筛选克隆：a）随机筛选；b）与大核DNA杂交筛选；c）与大核DNA克隆杂交筛选。其中一组克隆仅包含单一序列DNA，并且所有这些克隆的序列都与大核序列同源。与这些小核克隆具有同源序列的大核基因数量表明，大核序列在小核基因组中是成簇分布的。许多小核克隆包含重复DNA序列，并与总小核DNA的众多EcoRI片段杂交，产生相似但不完全相同的图谱。一些含有这些重复序列的小核克隆还包含与大核序列杂交的单一序列DNA。这些克隆定义了小核基因组中的一个主要散布重复序列家族，该家族在大核基因组形成过程中被消除。
Zotero 插件