肺炎克雷伯氏菌的固氮酶。在乙烯存在下酶周转过程中观察到的一个电子顺磁共振信号与铁钼辅因子有关。
Nitrogenase from Klebsiella pneumoniae. An e.p.r. signal observed during enzyme turnover under ethylene is associated with the iron-molybdenum cofactor.
作者信息
Hawkes T R, Lowe D J, Smith B E
出版信息
Biochem J. 1983 May 1;211(2):495-7. doi: 10.1042/bj2110495.
Abstract
During turnover at 10 degrees C at pH 7.4 in the presence of ethylene, the MoFe protein of Klebsiella pneumoniae nitrogenase (Kp 1) exhibited an electron-paramagnetic-resonance signal with g-values at 2.12, 1.998 and 1.987. 57Fe isotopic substitution demonstrated that this signal arose from the Kp 1 FeMo-cofactor in an S = 1/2 spin state.
摘要
在10摄氏度、pH值为7.4且存在乙烯的条件下进行周转时，肺炎克雷伯氏菌固氮酶（Kp 1）的钼铁蛋白呈现出电子顺磁共振信号，其g值分别为2.12、1.998和1.987。57Fe同位素取代表明该信号源自处于S = 1/2自旋态的Kp 1铁钼辅因子。
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