人MG63细胞干扰素的生产与纯化。
Production and purification of human MG63 cell interferon.
作者信息
Watanabe Y
出版信息
Microbiol Immunol. 1983;27(5):433-44. doi: 10.1111/j.1348-0421.1983.tb00602.x.
Abstract
MG63 cells induced by Sendai virus were a convenient source of human interferon, which was of the beta type in antigenicity. When analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), the interferon migrated as a single component with a molecular weight of 22,000 daltons. It was purified to apparent homogeneity by chromatography on Blue Sepharose, followed by SDS-PAGE, and estimated to have a specific activity of 4 x 10(8) international units/mg of protein.
摘要
仙台病毒诱导的MG63细胞是人类干扰素的便利来源，其抗原性为β型。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析时，该干扰素以单一成分迁移，分子量为22,000道尔顿。通过Blue Sepharose柱层析，随后进行SDS-PAGE，将其纯化至表观均一，估计其比活性为4×10⁸国际单位/毫克蛋白质。
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