Isom H C, Gao M, Wigdahl B
J Virol. 1984 Feb;49(2):426-36. doi: 10.1128/JVI.49.2.426-436.1984.
The genome of guinea pig cytomegalovirus (GPCMV) was analyzed and compared with that of human cytomegalovirus (HCMV). GPCMV and HCMV DNAs were isolated from virions and further purified by CsCl centrifugation. Purified GPCMV DNA sedimented as a single peak in a neutral sucrose gradient and was infectious when transfected into guinea pig embryo fibroblast cells. The cytopathology was characteristic of that seen after infection with GPCMV. Virus DNA purified from virions isolated from infected GPEF or 104C1 cells had a CsCl buoyant density of 1.713 g/cm3, which corresponds to a guanine plus cytosine content of 54.1%. The CsCl buoyant density of GPCMV DNA was slightly less than that of HCMV DNA (1.716 g/cm3), but sufficiently different so that the two virus DNA peaks did not coincide. GPCMV DNA cosedimented with T4 DNA in a neutral sucrose gradient. Restriction endonuclease cleavage of GPCMV or HCMV DNAs with HindIII, XbaI, or EcoRI yielded fragments easily separable by agarose gel electrophoresis and ranging from 1.0 X 10(6) to 25.8 X 10(6) daltons. The number, size, and molarity of GPCMV DNA fragments generated by restriction enzymes were determined. Hybridization of restriction endonuclease-cleaved GPCMV DNA with radioactively labeled HCMV DNA and, conversely, hybridization of restriction endonuclease-cleaved HCMV DNA with radioactively labeled GPCMV DNA indicated sequence homology between the two virus DNAs.
对豚鼠巨细胞病毒（GPCMV）的基因组进行了分析，并与人类巨细胞病毒（HCMV）的基因组进行了比较。从病毒粒子中分离出GPCMV和HCMV DNA，并通过CsCl离心进一步纯化。纯化的GPCMV DNA在中性蔗糖梯度中以单峰形式沉降，当转染到豚鼠胚胎成纤维细胞中时具有感染性。细胞病理学表现为感染GPCMV后所见的特征。从感染的豚鼠胚胎成纤维细胞（GPEF）或104C1细胞中分离出的病毒粒子纯化的病毒DNA，其CsCl浮力密度为1.713 g/cm3，对应鸟嘌呤加胞嘧啶含量为54.1%。GPCMV DNA的CsCl浮力密度略低于HCMV DNA（1.716 g/cm3），但差异足以使两个病毒DNA峰不重合。GPCMV DNA在中性蔗糖梯度中与T4 DNA共沉降。用HindIII、XbaI或EcoRI对GPCMV或HCMV DNA进行限制性内切酶切割，产生的片段可通过琼脂糖凝胶电泳轻松分离，大小范围为1.0×10(6)至25.8×10(6)道尔顿。测定了限制性内切酶产生的GPCMV DNA片段的数量、大小和摩尔浓度。用放射性标记的HCMV DNA与限制性内切酶切割的GPCMV DNA杂交，反之，用放射性标记的GPCMV DNA与限制性内切酶切割的HCMV DNA杂交，表明两种病毒DNA之间存在序列同源性。
