地理因素对溶组织内阿米巴致病酶谱分布的影响：印度酶谱XIV型的鉴定

Influence of geographical factors in the distribution of pathogenic zymodemes of Entamoeba histolytica: identification of zymodeme XIV in India.

作者信息

Sargeaunt P G, Baveja U K, Nanda R, Anand B S

出版信息

Trans R Soc Trop Med Hyg. 1984;78(1):96-101. doi: 10.1016/0035-9203(84)90185-8.

Abstract

Stocks of Entamoeba histolytica isolated and maintained in a variety of culture media, both axenic and polyxenic, were compared with each other and with strains previously characterized. The present stocks were isolated from subjects living in various cities in India. Using the enzyme patterns of: E C 5319 glucose phosphate isomerase (GPI); E C 11140 L-malate: NADP+ oxidoreductase (oxaloacetate decarboxylating) (ME): E C 2751 phosphoglucomutase (PGM); and E C 2711 hexakinase (HK), developed after electrophoresis, three zymodemes were identified in 54 individual isolations of E. histolytica. Most importantly only one zymodeme associated with pathogenicity occurred, identified 28 times, among the collection, one of which was from a liver abscess.

摘要

对在多种培养基（包括无菌培养基和多菌培养基）中分离并保存的溶组织内阿米巴菌株进行了相互比较，并与先前已鉴定特征的菌株进行了比较。目前的菌株是从生活在印度各个城市的受试者中分离出来的。利用电泳后得出的以下几种酶的模式：E.C.5319葡萄糖磷酸异构酶（GPI）；E.C.11140 L-苹果酸：NADP+氧化还原酶（草酰乙酸脱羧）（ME）；E.C.2751磷酸葡萄糖变位酶（PGM）；以及E.C.2711己糖激酶（HK），在54株溶组织内阿米巴的单独分离物中鉴定出了三种酶型。最重要的是，在收集的菌株中，仅出现了一种与致病性相关的酶型，共鉴定出28次，其中一次分离自肝脓肿。