Herrmann B
Virologie. 1983 Jan-Mar;34(1):19-24.
A mutant causing small plaques in chick embryo cells was isolated from influenza virus A/PR8/34 (H1N1). The diameter of the plaques remained constant over 39 passages. Virus titers in 8-11-day-old chick embryos were so low, that positive hemagglutination results could be recorded only in 35% of the inoculated eggs. There was no increase in virus titer after repeated passages in chick embryos. The sensitivity of the mutant to nonspecific serum and tissue inhibitors was higher than that of the initial strain. Mice survived intranasal inoculation with the mutant. The immunofluorescent antibody technique allowed the visualization of virus antigen in the lung of infected mice.
从甲型流感病毒A/PR8/34（H1N1）中分离出一种在鸡胚细胞中形成小蚀斑的突变体。蚀斑直径在39代传代过程中保持恒定。8至11日龄鸡胚中的病毒滴度很低，以至于仅在35%的接种卵中能记录到阳性血凝结果。在鸡胚中反复传代后病毒滴度没有增加。该突变体对非特异性血清和组织抑制剂的敏感性高于原始毒株。小鼠经鼻内接种该突变体后存活。免疫荧光抗体技术可使感染小鼠肺中的病毒抗原可视化。
