Comparison of liver alcohol dehydrogenases in Fischer-344 and Sprague-Dawley rats.

Livers of Sprague-Dawley rats contain 30-100% more alcohol dehydrogenase activity than livers of Fischer-344 rats. When weight-matched rats from both strains are injected with the same dose of ethanol (1.2 g/kg), Sprague-Dawley rats achieve lower blood alcohol levels than Fischer-344 rats at all the time-points tested. Purified alcohol dehydrogenases from both strains of rats exhibit identical electrophoretic mobilities in SDS-polyacrylamide Section and in isoelectric focusing slab gels, pH optima, peptide maps, Km for ethanol, and capacities to bind monospecific rabbit antibodies. Quantitative differences in alcohol dehydrogenase activity between these strains of rats are due to differences in their liver alcohol dehydrogenase levels.