Lonsdale D M, Hodge T P, Howe C J, Stern D B
Cell. 1983 Oct;34(3):1007-14. doi: 10.1016/0092-8674(83)90558-5.
The mitochondrial genome of maize contains a DNA sequence homologous to the chloroplast gene coding for the large subunit of ribulose-1,5-bisphosphate carboxylase (LS gene). The presence in mitochondrial DNA of both coding and flanking sequences of this gene has been demonstrated first, by cross hybridization between the purified organelle DNAs and between cloned mitochondrial and chloroplast DNA sequences and second, by in vitro transcription-translation of cloned mitochondrial DNA in an E. coli cell free system where a 21,000 dalton polypeptide is synthesized that can be precipitated with antibodies to wheat ribulose-1,5-bisphosphate carboxylase. In contrast to the 12 kb chloroplast homologous sequence found in the mitochondrial genome (Stern and Lonsdale, 1982), the sequence homologous to the LS gene is unaltered in mitochondrial DNA isolated from the male sterile cytoplasms of maize. The LS gene homologous sequence in the mitochondrial genome is located some 65 kb from the 18S mitochondrial rRNA gene and approximately 20 kb from the mitochondrial DNA sequence having homology to the chloroplast 16S rRNA gene.
玉米的线粒体基因组包含一段与叶绿体基因同源的DNA序列，该叶绿体基因编码核酮糖-1,5-二磷酸羧化酶的大亚基（LS基因）。首先，通过纯化的细胞器DNA之间以及克隆的线粒体和叶绿体DNA序列之间的交叉杂交，其次，通过在大肠杆菌无细胞系统中对克隆的线粒体DNA进行体外转录-翻译，已证明该基因的编码序列和侧翼序列存在于线粒体DNA中，在该系统中合成了一种21,000道尔顿的多肽，该多肽可用针对小麦核酮糖-1,5-二磷酸羧化酶的抗体沉淀。与线粒体基因组中发现的12 kb叶绿体同源序列（斯特恩和朗斯代尔，1982年）不同，从玉米雄性不育细胞质中分离的线粒体DNA中与LS基因同源的序列未发生改变。线粒体基因组中的LS基因同源序列位于距18S线粒体rRNA基因约65 kb处，距与叶绿体16S rRNA基因具有同源性的线粒体DNA序列约20 kb处。
