从大鼠吉田腹水肉瘤中分离胞苷二磷酸还原酶。

Separation of cytidine diphosphate reductase from rat Yoshida ascites sarcoma.

作者信息

Morita T, Murayama J, Shimizu N, Moriyama Y, Fujimura S

出版信息

Biochem Biophys Res Commun. 1984 Jan 13;118(1):212-6. doi: 10.1016/0006-291x(84)91088-x.

Abstract

CDP reductase was separated from the cytosol of rat Yoshida ascites sarcoma. The precipitate, which resulted from the acidification of the cytosol by acetic acid at pH 5.2, catalyzed specifically the reduction of CDP, whereas the concurrently resulted supernatant catalyzed those of UDP, ADP and GDP. The CDP reductase showed a single peak in the pattern of the enzyme activity in DEAE-cellulose and also in Sepharose 4B column chromatography with adequate recovery of the activity.

摘要

CDP还原酶是从大鼠吉田腹水肉瘤的胞质溶胶中分离出来的。通过在pH 5.2用乙酸将胞质溶胶酸化而产生的沉淀物特异性地催化CDP的还原，而同时产生的上清液催化UDP、ADP和GDP的还原。在DEAE-纤维素以及琼脂糖4B柱色谱的酶活性模式中，CDP还原酶呈现出单一峰，并且酶活性有足够的回收率。