将大肠杆菌不耐热肠毒素储存在滤纸上，用于通过免疫溶血反应进行检测。
Storage of Escherichia coli thermolabile enterotoxin on filter papers for assay by immune haemolysis reactions.
作者信息
Ricci L C, Fontes C F, Siqueira P S, Pestana de Castro A F
出版信息
Med Microbiol Immunol. 1984;172(4):243-8. doi: 10.1007/BF02123719.
PMID:6371466
Abstract
Thermolabile (LT) enterotoxin was prepared from stationary and shaken cultures of enterotoxigenic Escherichia coli (ETEC) grown in casamino acids-yeast extract medium and dried onto filter discs. These were then examined by a modification of the single radial immune haemolysis (SRIH) test. It was observed that LT antigenicity, as detected by this test, remained unaltered for as long as 30 days at room temperature.
摘要
不耐热（LT）肠毒素是从在酪蛋白氨基酸-酵母提取物培养基中生长的产肠毒素大肠杆菌（ETEC）的静置培养物和振荡培养物中制备的，并干燥在滤盘上。然后通过单径向免疫溶血（SRIH）试验的改良方法对其进行检测。观察到，通过该试验检测到的LT抗原性在室温下长达30天保持不变。
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