慢性促黄体生成素释放激素（LHRH）处理后大鼠睾丸匀浆对[4-14C]睾酮及其前体的代谢

Metabolism of [4-14C]testosterone and precursors by homogenates of rat testes after chronic LHRH-treatment.

作者信息

Eechaute W, Lacroix E, Leusen I

出版信息

J Steroid Biochem. 1984 May;20(5):1201-5. doi: 10.1016/0022-4731(84)90367-4.

Abstract

Adult male rats were injected daily for 8 days with an LHRH agonist. Twenty-four hours after the last injection testes-homogenates were incubated in the presence of a 4-14C-labeled steroid, either progesterone, 17 alpha-hydroxyprogesterone, dehydroepiandrosterone, androstenedione or testosterone. The activity of several enzymes involved in the androgen biosynthetic pathway was inferred from the amount of metabolites produced under these conditions. After LHRH-treatment a significant increase in the 17,20-lyase activity was observed without any significant change in the activity of 17 alpha-hydroxylase, 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase and 17 beta-hydroxysteroid dehydrogenase. The results of the experiments indicate that the decreased testosterone secretion observed in rats after chronic LHRH-administration is not due to an inhibition of the enzyme-systems studied.

摘要

成年雄性大鼠连续8天每天注射促性腺激素释放激素（LHRH）激动剂。最后一次注射24小时后，将睾丸匀浆在4-14C标记的类固醇（孕酮、17α-羟基孕酮、脱氢表雄酮、雄烯二酮或睾酮）存在的情况下进行孵育。从这些条件下产生的代谢物量推断雄激素生物合成途径中几种酶的活性。LHRH治疗后，观察到17,20-裂解酶活性显著增加，而17α-羟化酶、3β-羟基类固醇脱氢酶/δ5-δ4-异构酶和17β-羟基类固醇脱氢酶的活性没有任何显著变化。实验结果表明，慢性给予LHRH后大鼠睾丸酮分泌减少并非由于所研究的酶系统受到抑制。