酿酒酵母中核糖体前体RNA加工缺陷。
Defective processing of ribosomal precursor RNA in Saccharomyces cerevisiae.
作者信息
Mitlin J A, Cannon M
出版信息
Biochem J. 1984 Jun 1;220(2):461-7. doi: 10.1042/bj2200461.
Abstract
Saccharomyces cerevisiae (strain A224A) has an abnormal distribution of cytoplasmic ribosomal subunits when grown at 36 degrees C, with sucrose-gradient analysis of extracts revealing an apparent excess of material sedimenting at 60 S. This abnormality is not observed at either 23 degrees C or 30 degrees C. At 36 degrees C the defect(s) is expressed as a slowed conversion of 20 S ribosomal precursor RNA to mature 18 S rRNA, although the corresponding maturation of 27 S ribosomal precursor RNA to mature 25 S rRNA is normal. Studies on this yeast strain and on mutants derived from it may help to elucidate the role(s) of individual ribosomal components in controlling ribosome biogenesis in eukaryotes.
摘要
酿酒酵母（菌株A224A）在36℃培养时，细胞质核糖体亚基分布异常，对提取物进行蔗糖梯度分析显示，60S处有明显过量的沉降物质。在23℃或30℃时未观察到这种异常。在36℃时，缺陷表现为20S核糖体前体RNA向成熟18S rRNA的转化减慢，尽管27S核糖体前体RNA向成熟25S rRNA的相应成熟过程正常。对该酵母菌株及其衍生突变体的研究可能有助于阐明单个核糖体成分在真核生物核糖体生物合成控制中的作用。
相似文献
1
Defective processing of ribosomal precursor RNA in Saccharomyces cerevisiae.
Biochem J. 1984 Jun 1;220(2):461-7. doi: 10.1042/bj2200461.
2
RRP1, a Saccharomyces cerevisiae gene affecting rRNA processing and production of mature ribosomal subunits.
J Bacteriol. 1987 Apr;169(4):1571-8. doi: 10.1128/jb.169.4.1571-1578.1987.
3
Immature large ribosomal subunits containing the 7S pre-rRNA can engage in translation in Saccharomyces cerevisiae.
RNA Biol. 2015;12(8):838-46. doi: 10.1080/15476286.2015.1058477.
4
Ribosomal precursor RNA metabolism and cell division in the yeast Saccharomyces cerevisiae.
Mol Gen Genet. 1980;178(2):357-60. doi: 10.1007/BF00270484.
5
Maturation of ribosomal precursor RNA in Saccharomyces cerevisiae. A mutant with a defect in both the transport and terminal processing of the 20 S species.
J Mol Biol. 1980 Oct 25;143(2):179-99. doi: 10.1016/0022-2836(80)90197-7.
6
Domain III of Saccharomyces cerevisiae 25 S ribosomal RNA: its role in binding of ribosomal protein L25 and 60 S subunit formation.
J Mol Biol. 2000 Feb 11;296(1):7-17. doi: 10.1006/jmbi.1999.3432.
7
Impact of two neighbouring ribosomal protein clusters on biogenesis factor binding and assembly of yeast late small ribosomal subunit precursors.
PLoS One. 2019 Jan 17;14(1):e0203415. doi: 10.1371/journal.pone.0203415. eCollection 2019.
8
Mak16p is required for the maturation of 25S and 5.8S rRNAs in the yeast Saccharomyces cerevisiae.
Yeast. 2006 May;23(7):495-506. doi: 10.1002/yea.1368.
9
Processing of precursors of 21S ribosomal RNA from yeast mitochondria.
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1417-21. doi: 10.1073/pnas.77.3.1417.
10
Mutations of EXOSC3/Rrp40p associated with neurological diseases impact ribosomal RNA processing functions of the exosome in .
RNA. 2017 Apr;23(4):466-472. doi: 10.1261/rna.060004.116. Epub 2017 Jan 4.
引用本文的文献
1
A functional connection between translation elongation and protein folding at the ribosome exit tunnel in Saccharomyces cerevisiae.
Nucleic Acids Res. 2021 Jan 11;49(1):206-220. doi: 10.1093/nar/gkaa1200.
2
Role of the 40S beak ribosomal protein eS12 in ribosome biogenesis and function in .
RNA Biol. 2020 Sep;17(9):1261-1276. doi: 10.1080/15476286.2020.1767951. Epub 2020 Jun 7.
3
RRP1, a Saccharomyces cerevisiae gene affecting rRNA processing and production of mature ribosomal subunits.
J Bacteriol. 1987 Apr;169(4):1571-8. doi: 10.1128/jb.169.4.1571-1578.1987.
4
Application of high-performance liquid chromatography to the purification and characterization of ribosomal protein L3 from trichodermin-resistant yeast mutants.
Biochem J. 1986 Jul 15;237(2):421-6. doi: 10.1042/bj2370421.
5
Characterization of Saccharomyces cerevisiae strains displaying high-level or low-level resistance to trichothecene antibiotics.
Biochem J. 1990 May 1;267(3):709-13. doi: 10.1042/bj2670709.
6
A temperature sensitive mutant of Saccharomyces cerevisiae defective in pre-rRNA processing.
Nucleic Acids Res. 1991 Sep 25;19(18):5059-64. doi: 10.1093/nar/19.18.5059.
本文引用的文献
1
Eukaryotic ribosomal proteins. Two-dimensional electrophoretic studies.
FEBS Lett. 1971 Sep 15;17(1):163-167. doi: 10.1016/0014-5793(71)80588-4.
2
The nucleotide sequence of the intergenic region between the 5.8S and 26S rRNA genes of the yeast ribosomal RNA operon. Possible implications for the interaction between 5.8S and 26S rRNA and the processing of the primary transcript.
Nucleic Acids Res. 1981 Oct 10;9(19):4847-62. doi: 10.1093/nar/9.19.4847.
3
Cloning of yeast gene for trichodermin resistance and ribosomal protein L3.
Proc Natl Acad Sci U S A. 1981 Jan;78(1):238-42. doi: 10.1073/pnas.78.1.238.
4
Maturation of ribosomal precursor RNA in Saccharomyces cerevisiae. A mutant with a defect in both the transport and terminal processing of the 20 S species.
J Mol Biol. 1980 Oct 25;143(2):179-99. doi: 10.1016/0022-2836(80)90197-7.
5
A trichodermin-resistant mutant of Saccharomyces cerevisiae with an abnormal distribution of native ribosomal subunits.
Eur J Biochem. 1980;107(1):173-83. doi: 10.1111/j.1432-1033.1980.tb04638.x.
6
Studies on the structure and biogenesis of yeast ribosomes.
Biochem Soc Symp. 1982;47:79-93.
7
Ribosome structure, maturation of ribosomal RNA and drug sensitivity in temperature-sensitive mutants of Saccharomyces cerevisiae.
Mol Gen Genet. 1982;188(3):384-91. doi: 10.1007/BF00330038.
8
Intermediates in the assembly of ribosomes by a mutant of Escherichia coli.
Biochem J. 1980 Jul 15;190(1):157-70. doi: 10.1042/bj1900157.
9
Effects of cycloheximide on ribosomal RNA synthesis in yeast.
Biochem Biophys Res Commun. 1969 Feb 21;34(4):488-94. doi: 10.1016/0006-291x(69)90408-2.
10
Characteristics of the methylation in vivo of ribosomal RNA in yeast.
Biochim Biophys Acta. 1969 Dec 16;195(2):370-80. doi: 10.1016/0005-2787(69)90643-1.
Zotero 插件