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单克隆抗体在酶联免疫吸附测定(ELISA)中用于检测B型肉毒杆菌毒素。

Use of monoclonal antibodies in enzyme linked immunosorbent assay (ELISA) for detection of botulinum type B toxins.

作者信息

Notermans S, Kozaki S, Kamata Y, Sakaguchi G

出版信息

Jpn J Med Sci Biol. 1984 Jun;37(3):137-40. doi: 10.7883/yoken1952.37.137.

DOI:10.7883/yoken1952.37.137
PMID:6389944
Abstract

Use of polyclonal antibodies failed to correlate mouse assay with enzyme linked immunosorbent assay (ELISA) in titration of culture fluid of different strains of Clostridium botulinum type B. If ELISA is performed with such a monoclonal antibody that is capable of neutralizing the toxin, however, the lethal toxicity can be determined quantitatively.

摘要

在对不同B型肉毒梭菌菌株的培养液进行滴定分析时,使用多克隆抗体无法使小鼠试验结果与酶联免疫吸附测定法(ELISA)相关联。然而,如果使用能够中和毒素的单克隆抗体进行ELISA,就可以对致死毒性进行定量测定。

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引用本文的文献

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Toxins (Basel). 2017 Jan 18;9(1):38. doi: 10.3390/toxins9010038.
2
Sensitive enzyme-linked immunosorbent assay for detection of Clostridium botulinum neurotoxins A, B, and E using signal amplification via enzyme-linked coagulation assay.通过酶联凝血测定进行信号放大的用于检测肉毒梭菌神经毒素A、B和E的灵敏酶联免疫吸附测定。
J Clin Microbiol. 1993 Sep;31(9):2402-9. doi: 10.1128/jcm.31.9.2402-2409.1993.
3
Molecular cloning of the Clostridium botulinum structural gene encoding the type B neurotoxin and determination of its entire nucleotide sequence.
编码B型肉毒杆菌神经毒素的肉毒梭菌结构基因的分子克隆及其全核苷酸序列的测定。
Appl Environ Microbiol. 1992 Aug;58(8):2345-54. doi: 10.1128/aem.58.8.2345-2354.1992.