单克隆抗体在酶联免疫吸附测定（ELISA）中用于检测B型肉毒杆菌毒素。
Use of monoclonal antibodies in enzyme linked immunosorbent assay (ELISA) for detection of botulinum type B toxins.
作者信息
Notermans S, Kozaki S, Kamata Y, Sakaguchi G
出版信息
Jpn J Med Sci Biol. 1984 Jun;37(3):137-40. doi: 10.7883/yoken1952.37.137.
PMID:6389944
Abstract
Use of polyclonal antibodies failed to correlate mouse assay with enzyme linked immunosorbent assay (ELISA) in titration of culture fluid of different strains of Clostridium botulinum type B. If ELISA is performed with such a monoclonal antibody that is capable of neutralizing the toxin, however, the lethal toxicity can be determined quantitatively.
摘要
在对不同B型肉毒梭菌菌株的培养液进行滴定分析时，使用多克隆抗体无法使小鼠试验结果与酶联免疫吸附测定法（ELISA）相关联。然而，如果使用能够中和毒素的单克隆抗体进行ELISA，就可以对致死毒性进行定量测定。
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