Lindenschmidt E G
Immun Infekt. 1984 Apr;12(2):94-100.
Rheumatoid factors (RF) are autoantibodies mainly directed against autologous IgG. They belong at most to the IgM class antibodies. It is demonstrated at groups with unsolved hepatitis B, rubella, syphilis and toxoplasmose infection that RF do occur not rarely at these patients even without rheumatoid arthritis. This is probably due to stimulation by antigen-IgG-complexes. During serologic detection of specific IgM antibodies they present an antigen independent mu-specificity. So the test for specific IgM might even loose its diagnostic and possibly therapy indicating value. It is shown how the disturbance by RF can be calculated after adsorption with aggregated IgG. Also RF can be titrated by an enzyme immunoassay (ELISA). With IgG coated latex particles RF can be eliminated prior to the IgM-test. Solid phase techniques which are applied with enzyme-coupled antigen instead of marked anti-IgM cannot be disturbed by RF significantly.
类风湿因子（RF）是主要针对自身IgG的自身抗体。它们大多属于IgM类抗体。在未解决的乙型肝炎、风疹、梅毒和弓形虫感染人群中已证实，即使没有类风湿性关节炎，这些患者中RF也不少见。这可能是由于抗原-IgG复合物的刺激。在特异性IgM抗体的血清学检测过程中，它们呈现出一种与抗原无关的μ特异性。因此，特异性IgM检测甚至可能失去其诊断及可能的治疗指示价值。文中展示了如何通过用聚集的IgG吸附后计算RF造成的干扰。此外，RF可以通过酶免疫测定法（ELISA）进行滴定。使用包被IgG的乳胶颗粒，可以在IgM检测之前消除RF。应用酶偶联抗原而非标记抗IgM的固相技术不会受到RF的显著干扰。
