从各种亚类的小鼠IgG制备F(ab')2片段。
Preparation of F(ab')2 fragments from mouse IgG of various subclasses.
作者信息
Lamoyi E, Nisonoff A
出版信息
J Immunol Methods. 1983 Jan 28;56(2):235-43. doi: 10.1016/0022-1759(83)90415-5.
We have investigated the effects of peptic digestion on mouse monoclonal immunoglobulins of each subclass of IgG. F(ab')2 fragments were obtained in good yield from IgG1, IgG2a and IgG3 proteins. The relative rates of digestion were IgG3 greater than IgG2a greater than IgG1. Variations in rate of digestion were noted for individual monoclonal IgG3 proteins. Five different IgG2b proteins were degraded very rapidly without the detectable formation of antigen-binding F(ab')2 fragments. One IgG1 protein out of four tested was also rapidly degraded.
我们研究了胃蛋白酶消化对小鼠 IgG 各亚类单克隆免疫球蛋白的影响。从 IgG1、IgG2a 和 IgG3 蛋白中以高产率获得了 F(ab')2 片段。消化的相对速率为 IgG3 大于 IgG2a 大于 IgG1。注意到个别单克隆 IgG3 蛋白的消化速率存在差异。五种不同的 IgG2b 蛋白降解非常迅速，未检测到抗原结合性 F(ab')2 片段的形成。所测试的四种 IgG1 蛋白中有一种也迅速降解。
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