Probst B
Acta Endocrinol (Copenh). 1983 Aug;103(4):567-71. doi: 10.1530/acta.0.1030567.
The in vitro steroidogenic response of gerbil and mouse whole testes and isolated testicular interstitial cells to stimulation with gonadotrophic hormones was compared. The testosterone release during incubation in vitro with LH/FSH was measured radioimmunologically. In whole testes incubation, basal testosterone release after 4 h was significantly lower in gerbil as compared to mouse testes, but the testosterone release on maximal LH-stimulation was not significantly different. The increment in the testosterone production from basal to stimulated levels was greater in gerbil, the production per gram testis was greater in mouse testes. Mechanically dispersed interstitial cell preparations from gerbil and mouse released comparable amounts of testosterone during in vitro incubation. The lowest effective standard-concentration was 1.1 microIU LH/vial in gerbil and 35.9 microIU LH/vial in mouse interstitial cell incubations. Half-maximum testosterone release (ED50) occurred at 8-15 microU LH/vial for gerbil and 86-200 microIU LH/vial (ranges) for mouse interstitial cells. The difference in sensitivity to LH stimulation of gerbil and mouse interstitial cells is approximately 13-fold allowing the application of the gerbil interstitial cell assay to studies dealing with very small samples or little bioactive LH-concentrations.
比较了沙鼠和小鼠全睾丸及分离的睾丸间质细胞对促性腺激素刺激的体外类固醇生成反应。采用放射免疫法测定体外与促黄体生成素/促卵泡生成素孵育期间的睾酮释放量。在全睾丸孵育中，沙鼠睾丸孵育4小时后的基础睾酮释放量显著低于小鼠睾丸，但最大促黄体生成素刺激后的睾酮释放量无显著差异。从基础水平到刺激水平，沙鼠睾酮产量的增加幅度更大，每克睾丸的产量小鼠睾丸更高。沙鼠和小鼠机械分散的间质细胞制剂在体外孵育期间释放的睾酮量相当。沙鼠间质细胞孵育中最低有效标准浓度为1.1微国际单位促黄体生成素/管，小鼠间质细胞孵育中为35.9微国际单位促黄体生成素/管。沙鼠间质细胞半数最大睾酮释放量（ED50）出现在8 - 15微单位促黄体生成素/管，小鼠间质细胞为86 - 200微国际单位促黄体生成素/管（范围）。沙鼠和小鼠间质细胞对促黄体生成素刺激的敏感性差异约为13倍，这使得沙鼠间质细胞测定法可应用于处理非常小的样本或低生物活性促黄体生成素浓度的研究。
