Gorman S P, Scott E M, Hutchinson E P
J Appl Bacteriol. 1984 Aug;57(1):153-63. doi: 10.1111/j.1365-2672.1984.tb02368.x.
The emergence and development of chemical and thermal resistance in spores of Bacillus subtilis was examined. The chemicals studied were of the disinfectant type: glutaraldehyde, hypochlorite, hypochlorite-methanol and povidone-iodine. Growth and sporulation were followed by electron microscopy and resistance assigned to specific stages in relation to 45Ca and DPA accumulation. A sequential development of resistance was observed with thermal resistance appearing first at early Stage V corresponding to maturation of cortex and deposition of rudimentary spore coat material. Chemical resistance coincided with middle to late Stage V dependent on the chemical concerned. A progressive development of resistance was observed on prolonged incubation in sporulation medium and was affected by inclusion of lysozyme in the spore washing sequence.
对枯草芽孢杆菌孢子中化学抗性和热抗性的出现与发展进行了研究。所研究的化学物质属于消毒剂类型：戊二醛、次氯酸盐、次氯酸盐 - 甲醇和聚维酮碘。通过电子显微镜观察生长和芽孢形成过程，并根据与45Ca和二吡啶二酸（DPA）积累相关的特定阶段来确定抗性。观察到抗性呈顺序发展，热抗性首先出现在V期早期，对应于皮层成熟和基本芽孢衣物质的沉积。化学抗性则取决于所涉及的化学物质，出现在V期中后期。在芽孢形成培养基中长时间培养时，观察到抗性逐渐发展，并且在孢子洗涤过程中加入溶菌酶会对其产生影响。
