人外周血单个核细胞培养中抗体合成的抗原特异性诱导与调节
Antigen-specific induction and regulation of antibody synthesis in cultures of human peripheral blood mononuclear cells.
作者信息
Hoffmann M K
出版信息
Proc Natl Acad Sci U S A. 1980 Feb;77(2):1139-43. doi: 10.1073/pnas.77.2.1139.
Abstract
Culture conditions suitable for specific sensitization of human peripheral blood lymphocytes were established. Production of antibody to sheep erythrocytes and to the 2,4,6-trinitrophenyl group conjugated to burro erythrocytes was investigated in terms of cross-sensitization, feedback control by antibody to the immunogen, and regulation by helper and suppressor T cells. Generation of antigen-specific antibody-forming cells was found to be restricted to a narrow concentration range of cultured peripheral blood lymphocytes. The data suggest that antibody formation is limited by suppressor T cells in high-cell-density cultures and by low frequencies of helper T cells in low-cell-density cultures.
摘要
建立了适合人类外周血淋巴细胞特异性致敏的培养条件。从交叉致敏、免疫原抗体的反馈控制以及辅助性T细胞和抑制性T细胞的调节方面，研究了针对绵羊红细胞以及与驴红细胞偶联的2,4,6 -三硝基苯基基团的抗体产生情况。发现抗原特异性抗体形成细胞的产生仅限于培养的外周血淋巴细胞的狭窄浓度范围。数据表明，在高细胞密度培养中抗体形成受抑制性T细胞限制，而在低细胞密度培养中受辅助性T细胞低频率的限制。
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