从日本临床病变中分离的柠檬酸杆菌中汞抗性R质粒的金属抗性及挥发活性调查

Survey of resistance to metals and volatilization activity of Hg-resistant R plasmids in Citrobacter isolated from clinical lesions in Japan.

作者信息

Nakahara H, Asakawa M, Yonekura I, Sato A, Ohshima K, Kitamura M, Kozukue H

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1984 Aug;257(3):400-8.

Abstract

Clinical isolates of Citrobacter (277 strains) were studied for the resistance to four metals and four drugs. The distribution patterns of their susceptibility to Hg, Cd and As clearly revealed two peaks, but only a single peak of resistance to Pb. The frequencies of resistance to Hg, Cd, As, SM, TC, CP and KM were 31.8, 94.2, 57.8, 51.3, 44.8, 35.7 and 28.9%, respectively. And we selected 88 mercury resistant strains, and they were tested for their ability to transfer the resistance to the mercury and drug sensitive recipients of E. coli K12-ML1410-Nx and E. coli JE17-Rif. Among 88 strains of Hg resistant Citrobacter, 80 R plasmids with Hg resistance could be demonstrated. It should be noted that transferable R plasmids with Hg resistance were demonstrated in 91% of the Hg-resistant isolates. Furthermore, we tested the volatilization of mercury by strains containing these mercury resistance plasmids, by using radioactive 203Hg2+. All of these isolates of Citrobacter have volatilization activity of Hg2+. Also, all of these volatilization activity is inducible.

摘要

对柠檬酸杆菌的临床分离株（277株）进行了对四种金属和四种药物的抗性研究。它们对汞、镉和砷的敏感性分布模式明显显示出两个峰值，但对铅的抗性只有一个峰值。对汞、镉、砷、链霉素、四环素、氯霉素和卡那霉素的抗性频率分别为31.8%、94.2%、57.8%、51.3%、44.8%、35.7%和28.9%。我们挑选了88株耐汞菌株，并检测了它们将抗性转移至大肠杆菌K12-ML1410-Nx和大肠杆菌JE17-Rif的汞和药物敏感受体的能力。在88株耐汞柠檬酸杆菌中，可证明有80个带有汞抗性的R质粒。值得注意的是，在91%的耐汞分离株中证明了带有汞抗性的可转移R质粒。此外，我们通过使用放射性203Hg2+检测了含有这些汞抗性质粒的菌株对汞的挥发情况。所有这些柠檬酸杆菌分离株都具有Hg2+的挥发活性。而且，所有这些挥发活性都是可诱导的。