脆弱拟杆菌表面抗原和菌体抗原的研究
A study of the surface and somatic antigens of Bacteroides fragilis.
作者信息
Elhag K M, Tabaqchali S
出版信息
J Hyg (Lond). 1978 Jun;80(3):439-49. doi: 10.1017/s0022172400024918.
Abstract
Antisera against live cultures of 20 strains of Bacteroides fragilis produced in rabbits agglutinated homologous whole-cell as well as O-antigen suspensions with several cross-reactions. Pure specific antisera were produced by absorbing cross-reacting antigens. O-antisera reacted against O-antigens but failed to do so against most whole-cell suspensions, suggesting the presence of surface structures which might have blocked the reactions. The titres of the whole-cell antisera were not significantly higher than those of O-antisera, but the reactions were more definite and easier to read. This system may prove useful in serological studies of B. fragilis.
摘要
用20株脆弱拟杆菌的活培养物在兔体内制备的抗血清，能凝集同源全细胞以及O抗原悬液，并出现几种交叉反应。通过吸收交叉反应抗原制备出了纯特异性抗血清。O抗血清能与O抗原发生反应，但对大多数全细胞悬液则无此反应，这表明存在可能阻碍反应的表面结构。全细胞抗血清的效价并不显著高于O抗血清，但反应更明确且更易于判读。该系统可能在脆弱拟杆菌的血清学研究中证明有用。
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