Yamada S, Lieber C S
J Clin Invest. 1984 Dec;74(6):2285-9. doi: 10.1172/JCI111656.
This investigation was performed to determine whether chronic ethanol feeding alters the lipid composition or the fluidity of liver plasma membranes. Male Sprague-Dawley rats were pair-fed nutritionally adequate liquid diets containing ethanol as 36% of energy or an isocaloric amount of carbohydrate for 4-5 wk. Contrasting with other membranes, chronic ethanol feeding resulted in an increase in hepatic plasma membrane fluidity as assessed by fluorescence anisotropy. This alteration was associated with a decrease in plasma membrane cholesterol content.
进行这项研究是为了确定长期摄入乙醇是否会改变肝细胞膜的脂质组成或流动性。将雄性斯普拉格-道利大鼠成对饲养,给予营养充足的液体饮食,其中乙醇提供36%的能量,或给予等热量的碳水化合物,持续4-5周。与其他膜不同,通过荧光各向异性评估发现,长期摄入乙醇会导致肝细胞膜流动性增加。这种改变与细胞膜胆固醇含量的降低有关。