Lovkova M Ia, Buzuk G N, Kliment'eva N I, Grinkevich N I
Prikl Biokhim Mikrobiol. 1983 Jul-Aug;19(4):560-3.
A new method for developing alkaloids of the piperidine, pyridine, isochinoline, indole, purine and chinoline groups in a thin sorbent layer has been developed. This method is based on the use of the Dragendorf reagent, subsequent disintegration of the stained complex, and chromatogram treatment with a sodium thiosulfate ammonia solution. The method makes it possible to measure quantitatively alkaloids of various structures, using UV-spectrometry after their elution. The relative error is 1.9-5.5% in three parallel measurements.