一种用于定量薄层色谱斑点的廉价的、基于微型计算机的视频光密度计。

An inexpensive, microcomputer-based, video densitometer for quantitating thin-layer chromatographic spots.

作者信息

Ford-Holevinski T S, Agranoff B W, Radin N S

出版信息

Anal Biochem. 1983 Jul 1;132(1):132-6. doi: 10.1016/0003-2697(83)90435-9.

Abstract

An inexpensive video densitometer based on an Apple II microcomputer using commercially available components was developed. The device was used to quantitate charred lipid and colored spots on thin-layer chromatography plates. The method is useful for lipid samples weighing 0.1 to 4 micrograms on high-performance plates and 1 to 20 micrograms on ordinary plates. The software automatically corrects for baseline drift due to dirt or nonuniformity in the plate.

摘要

开发了一种基于苹果II型微型计算机、使用市售组件的廉价视频密度计。该设备用于定量薄层色谱板上的烧焦脂质和色斑。该方法适用于高性能板上重0.1至4微克的脂质样品以及普通板上重1至20微克的脂质样品。该软件会自动校正由于板上有污垢或不均匀而导致的基线漂移。