单克隆抗溶菌酶抗体Fab片段与其抗原复合物的初步晶体学研究。

Preliminary crystallographic study of the complex between the Fab fragment of a monoclonal anti-lysozyme antibody and its antigen.

作者信息

Mariuzza R A, Janković D L, Boulot G, Amit A G, Saludjian P, Le Guern A, Mazié J C, Poljak R J

出版信息

J Mol Biol. 1983 Nov 15;170(4):1055-8. doi: 10.1016/s0022-2836(83)80206-x.

Abstract

Preliminary crystallographic data are given for the complex between the Fab fragment of a monoclonal anti-lysozyme antibody and its antigen. This crystalline complex was found by screening a number of Fab-lysozyme complexes prepared from monoclonal anti-lysozyme antibodies produced by hybrids of BALB/c immune spleen cells with a non-secreting mouse hybrid myeloma line. The complex crystallizes in the monoclinic space group P21 with a = 55.5 (+/- 0.1) A, b = 143.5 (+/- 0.3) A, c = 49.1 (+/- 0.1) A, beta = 120 degrees 20' (+/- 10'). X-ray photographs show reflections extending to a resolution of 2.7 A. The crystals are suitable for high-resolution X-ray diffraction studies.

摘要

给出了单克隆抗溶菌酶抗体的Fab片段与其抗原之间复合物的初步晶体学数据。通过筛选从BALB/c免疫脾细胞与非分泌型小鼠杂交骨髓瘤细胞系杂交产生的单克隆抗溶菌酶抗体制备的多种Fab-溶菌酶复合物，发现了这种晶体复合物。该复合物在单斜空间群P21中结晶，a = 55.5（±0.1）Å，b = 143.5（±0.3）Å，c = 49.1（±0.1）Å，β = 120°20′（±10′）。X射线照片显示反射延伸至2.7 Å的分辨率。这些晶体适用于高分辨率X射线衍射研究。