眼虫微粒体中1-酰基甘油磷酸和甘油磷酸酰基转移酶的比较

Comparison of 1-acylglycerophosphate and glycerophosphate acyltransferases from Euglena microsomes.

作者信息

Hershenson S, Ernst-Fonberg M L

出版信息

Int J Biochem. 1984;16(2):219-23. doi: 10.1016/0020-711x(84)90076-4.

Abstract

Both glycerophosphate and monoacylglycerophosphate acyltransferases from Euglena microsomes were inhibited by N-ethylmaleimide, but their responses to heat inactivation and sn-glyceraldehyde-3-phosphate differed. Glycerophosphate acyltransferase had a higher V with palmitoyl-CoA compared to oleoyl-CoA; the reverse was true for monoacylglycerophosphate acyltransferase. Km's (microM) for the glycerophosphate acyltransferase were: palmitoyl-CoA, 21; oleoyl-CoA, 14; and sn-glycerol-3-phosphate, 2900. Km's (microM) for monoacylglycerophosphate acyltransferase were: palmitoyl-CoA, 7; oleoyl-CoA, 4; and 1-palmitoyl-sn-glycerol-3-phosphate, 48.

摘要

来自眼虫微粒体的甘油磷酸酰基转移酶和单酰甘油磷酸酰基转移酶均受到N-乙基马来酰亚胺的抑制，但它们对热失活和3-磷酸甘油醛的反应有所不同。与油酰辅酶A相比，甘油磷酸酰基转移酶与棕榈酰辅酶A反应时具有更高的V；单酰甘油磷酸酰基转移酶则相反。甘油磷酸酰基转移酶的Km值（微摩尔）分别为：棕榈酰辅酶A，21；油酰辅酶A，14；以及3-磷酸甘油，2900。单酰甘油磷酸酰基转移酶的Km值（微摩尔）分别为：棕榈酰辅酶A，7；油酰辅酶A，4；以及1-棕榈酰-sn-甘油-3-磷酸，48。