Burns B G, Sangalang G B, Freeman H C, McMenemy M
Gen Comp Endocrinol. 1984 Jun;54(3):429-32. doi: 10.1016/0016-6480(84)90158-8.
Testosterone was isolated and quantified from male lobster serum and testes by solvent extraction, sequential thin-layer chromatography, and high-performance liquid chromatography. The identity of the isolated steroid was established by its isopolarity and isomorphicity with authentic radiolabeled testosterone and its acetate derivative. The concentrations of testosterone were determined by high-performance liquid chromatography, by a double-isotope derivative assay, and by radioimmunoassay. The concentrations of testosterone as determined by the three methods were the same and were 0.3 ng/ml and 14.3 ng/g in lobster serum and testes, respectively.
通过溶剂萃取、连续薄层色谱法和高效液相色谱法，从雄性龙虾的血清和睾丸中分离并定量了睾酮。通过与真实的放射性标记睾酮及其乙酸酯衍生物的等极性和同晶型确定了分离出的类固醇的身份。通过高效液相色谱法、双同位素衍生物测定法和放射免疫测定法测定睾酮的浓度。三种方法测定的睾酮浓度相同，龙虾血清和睾丸中的浓度分别为0.3 ng/ml和14.3 ng/g。
