Ballantyne J S, Storey K B
J Exp Zool. 1984 May;230(2):165-74. doi: 10.1002/jez.1402300202.
A method is presented for the isolation of mitochondria with good respiratory control from the hepatopancreas of the marine clam Mercenaria mercenaria. Palmitoyl-L-carnitine is the preferred substrate of the mitochondria of the hepatopancreas based on state 3 rates of oxidation (in the presence of ADP). Rates of oxidation of pyruvate and glutamate were about one-half that of the lipid substrate in state 3. alpha-Glycerophosphate was oxidized at a rate about one-third that of palmitoyl-L-carnitine. All Krebs cycle intermediates were oxidized to some extent. Proline was not oxidized at detectable levels. The optimal range of KCl concentrations for the oxidation of palmitoyl-L-carnitine is between 250 and 500 mM whereas the optimal range of KCl concentration for the oxidation of succinate is between 200 and 350 mM. The optimal range of pH for the oxidation of succinate and for the oxidation of palmitoyl-L-carnitine lies between pH 6.5 and 7.5 based on the respiratory control ratio.
本文介绍了一种从海蛤紫贻贝的肝胰腺中分离出具有良好呼吸控制能力的线粒体的方法。基于状态3氧化速率（在ADP存在下），棕榈酰-L-肉碱是肝胰腺线粒体的首选底物。在状态3下，丙酮酸和谷氨酸的氧化速率约为脂质底物的一半。α-甘油磷酸的氧化速率约为棕榈酰-L-肉碱的三分之一。所有三羧酸循环中间体都有一定程度的氧化。脯氨酸在可检测水平下未被氧化。棕榈酰-L-肉碱氧化的最佳KCl浓度范围在250至500 mM之间，而琥珀酸氧化的最佳KCl浓度范围在200至350 mM之间。基于呼吸控制率，琥珀酸氧化和棕榈酰-L-肉碱氧化的最佳pH范围在pH 6.5至7.5之间。
