克氏锥虫的糖缀合物、一种糖蛋白的纯化及组成
The glycoconjugates from Trypanosoma Cruzi, purification and composition of a glycoprotein.
作者信息
de Lederkremer R M, Waksman N, de Martini G J, Segura E L
出版信息
Comp Biochem Physiol B. 1982;72(2):313-6. doi: 10.1016/0305-0491(82)90052-9.
PMID:6749405
Abstract
- The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. The slower migrating glycoconjugate, a glycoprotein of molecular weight 45, 000 was purified to apparent homogeneity. 3. A content of 76% protein, 17% neutral sugars and 6% glucosamine was analysed. Galactose and mannose occur in a ratio of 3:1 as determined by gas-liquid chromatography. A trace of glucose was detected in some samples.
摘要
- 从源自图拉韦恩株的克氏锥虫非感染性系（To）的培养型上鞭毛体中用酚 - 水提取的糖缀合物复合物，经十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳仅显示出两个组分。2. 迁移较慢的糖缀合物，一种分子量为45,000的糖蛋白，被纯化至表观均一性。3. 分析得出其蛋白质含量为76%，中性糖含量为17%，氨基葡萄糖含量为6%。通过气相色谱法测定，半乳糖和甘露糖的比例为3:1。在一些样品中检测到微量葡萄糖。
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