纤细裸藻中L-抗坏血酸过氧化物酶对过氧化氢的代谢
Metabolism of hydrogen peroxide in Euglena gracilis Z by L-ascorbic acid peroxidase.
作者信息
Shigeoka S, Nakano Y, Kitaoka S
出版信息
Biochem J. 1980 Jan 15;186(1):377-80. doi: 10.1042/bj1860377.
Abstract
Euglena gracilis was found to contain a peroxidase that specifically require L-ascorbic acid as the natural electron donor in the cytosol. The presence of an oxidation-reduction system metabolizing L-ascorbic acid was demonstrated in Euglena cells. Oxidation of L-ascorbic acid by the peroxidase, and the absence of ascorbic acid oxidase activity, suggests that the system functions to remove H2O2 in E. gracilis, which lacks catalase.
摘要
人们发现纤细裸藻在其细胞质中含有一种过氧化物酶，该酶特别需要L-抗坏血酸作为天然电子供体。在裸藻细胞中证实存在代谢L-抗坏血酸的氧化还原系统。过氧化物酶对L-抗坏血酸的氧化作用以及抗坏血酸氧化酶活性的缺失表明，该系统在缺乏过氧化氢酶的纤细裸藻中发挥着清除过氧化氢的作用。
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本文引用的文献
1
Peroxidative Activity in Euglena gracilis.绿眼虫中的过氧化物酶活性。
Plant Physiol. 1975 Jun;55(6):1123-4. doi: 10.1104/pp.55.6.1123.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
The reduction of cytochrome c by photosynthetic pyridine nucleotide reductase and transhydrogenase.光合吡啶核苷酸还原酶和转氢酶对细胞色素c的还原作用。
Biochim Biophys Acta. 1962 Aug 13;62:417-20. doi: 10.1016/0006-3002(62)90273-1.
4
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
5
6
Ascorbic acid and photosynthesis. I. Monodehydroascorbic acid reductase of chloroplasts.抗坏血酸与光合作用。I. 叶绿体的单脱氢抗坏血酸还原酶。
Biochim Biophys Acta. 1958 Dec;30(3):453-7. doi: 10.1016/0006-3002(58)90089-1.
7
Ascorbic acid and glutathione as respiratory carriers in the respiration of pea seedlings.抗坏血酸和谷胱甘肽作为豌豆幼苗呼吸作用中的呼吸载体。
Biochem J. 1956 Feb;62(2):248-59. doi: 10.1042/bj0620248.
8
The occurrence and fine structural characterization of microbodies in Euglena gracilis.纤细裸藻中微体的发生及精细结构特征
Protoplasma. 1971;72(2):141-52. doi: 10.1007/BF01279047.
9
The intracellular localization of glycollate oxidoreductase in Euglena gracilis.乙醇酸氧化还原酶在纤细裸藻中的细胞内定位。
Biochem J. 1971 Sep;124(2):275-81. doi: 10.1042/bj1240275.
10
Phytochrome-mediated synthesis of ascorbic acid oxidase in mustard cotyledons.光敏色素介导的芥菜子叶中抗坏血酸氧化酶的合成。
Biochim Biophys Acta. 1974 Sep 5;362(2):258-65. doi: 10.1016/0304-4165(74)90218-9.
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