通过荧光显微镜监测活细胞中的相对线粒体膜电位。
Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy.
作者信息
Johnson L V, Walsh M L, Bockus B J, Chen L B
出版信息
J Cell Biol. 1981 Mar;88(3):526-35. doi: 10.1083/jcb.88.3.526.
Abstract
Permeant cationic fluorescent probes are shown to be selectively accumulated by the mitochondria of living cells. Mitochondria-specific interaction of such molecules is apparently dependent on the high trans-membrane potential (inside negative) maintained by functional mitochondria. Dissipation of the mitochondrial trans-membrane and potential by ionophores or inhibitors of electron transport eliminates the selective mitochondrial association of these compounds. The application of such potential-dependent probes in conjunction with fluorescence microscopy allows the monitoring of mitochondrial membrane potential in individual living cells. Marked elevations in mitochondria-associated probe fluorescence have been observed in cells engaged in active movement. This approach to the analysis of mitochondrial membrane potential should be of value in future investigations of the control of energy metabolism and energy requirements of specific biological functions at the cellular level.
摘要
渗透性阳离子荧光探针可被活细胞的线粒体选择性积累。这类分子与线粒体的特异性相互作用显然依赖于功能性线粒体维持的高跨膜电位（内膜为负）。离子载体或电子传递抑制剂使线粒体跨膜电位消散，会消除这些化合物与线粒体的选择性结合。将这种电位依赖性探针与荧光显微镜结合使用，可监测单个活细胞中的线粒体膜电位。在活跃运动的细胞中，已观察到与线粒体相关的探针荧光显著升高。这种分析线粒体膜电位的方法在未来细胞水平能量代谢控制和特定生物学功能能量需求的研究中应具有重要价值。
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本文引用的文献
1
Localization of mitochondria in living cells with rhodamine 123.
Proc Natl Acad Sci U S A. 1980 Feb;77(2):990-4. doi: 10.1073/pnas.77.2.990.
2
Fluorescent probes for conformational states of proteins. II. The binding of 2-p-toluidinylnaphthalene-6-sulfonate to alpha-chymotrypsin.
Biochemistry. 1967 Feb;6(2):559-66. doi: 10.1021/bi00854a025.
3
The interaction of a naphthalene dye with apomyoglobin and apohemoglobin. A fluorescent probe of non-polar binding sites.
J Mol Biol. 1965 Sep;13(2):482-95. doi: 10.1016/s0022-2836(65)80111-5.
4
Determination of membrane potentials in human and Amphiuma red blood cells by means of fluorescent probe.
J Physiol. 1974 Jun;239(3):519-52. doi: 10.1113/jphysiol.1974.sp010581.
5
Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles.
Biochemistry. 1974 Jul 30;13(16):3315-30. doi: 10.1021/bi00713a022.
6
The problem of cation-binding sites in the energized membrane of intact mitochondria.
Eur J Biochem. 1973 May 2;34(3):577-85. doi: 10.1111/j.1432-1033.1973.tb02798.x.
7
Conversion of biomembrane-produced energy into electric form. I. Submitochondrial particles.
Biochim Biophys Acta. 1970 Aug 4;216(1):1-12. doi: 10.1016/0005-2728(70)90153-2.
8
A fluorescence probe of energy-dependent structure changes in fragmented membranes.
Proc Natl Acad Sci U S A. 1969 Feb;62(2):612-9. doi: 10.1073/pnas.62.2.612.
9
Conversion of biomembrane-produced energy into electric form. II. Intact mitochondria.
Biochim Biophys Acta. 1970 Aug 4;216(1):13-21. doi: 10.1016/0005-2728(70)90154-4.
10
Age-related loss of DNA repair synthesis in isolated rat myocardial cells.
Exp Cell Res. 1975 Dec;96(2):412-6. doi: 10.1016/0014-4827(75)90276-1.
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